Sample Metadata and Comparison Design

Proteomics interpretation depends on knowing which samples belong to which biological groups.

Sample Metadata

A minimal metadata table should describe:

sample_id
condition
group
replicate
batch
comparison

Comparison Design

Before interpreting differential proteins, define the biological comparison clearly.

Examples:

treated_vs_control
disease_vs_healthy
timepoint_24h_vs_0h
resistant_vs_sensitive